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mouse myeloma sp2 o cells  (ATCC)


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    Structured Review

    ATCC mouse myeloma sp2 o cells
    Mouse Myeloma Sp2 O Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 763 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp2+o+mouse+myeloma+cells/Sp2/pmc08846386-186-4-8
    Average 96 stars, based on 763 article reviews
    mouse myeloma sp2 o cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    HAT Assay:

    Article Title: Blockade of the interaction between BMP9 and endoglin on erythroid progenitors promotes erythropoiesis in mice
    Article Snippet: .. Briefly, hybridomas were obtained by fusing the spleen cells of the immunized mouse and Sp2/o mouse myeloma cells (ATCC ID: CRL1581) with polyethylene glycol and maintained in DMEM (Invitrogen) containing 10% FBS, 50 μmol/L β‐mercaptoethanol, 50 μg/ml insulin, 10 ng/ml IL‐6 and HAT media supplement (Sigma). ..

    Article Title: Blockade of the interaction between BMP9 and endoglin on erythroid progenitors promotes erythropoiesis in mice.
    Article Snippet: .. Briefly, hybridomas were obtained by fusing the spleen cells of the immunized mouse and Sp2/o mouse myeloma cells (ATCC ID: CRL1581) with polyethylene glycol and maintained in DMEM (Invitrogen) containing 10% FBS, 50 μmol/L β-mercaptoethanol, 50 μg/mL insulin, 10 ng/mL IL-6 and HAT media supplement (Sigma). ..

    Stable Transfection:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: .. Materials and Methods SP2/O mouse myeloma cells were stably transfected with a construct encoding a λ6 LC (Wil) under control of the CMV promoter, while λ2-producing myeloma cell line RPMI 8226 was purchased from the ATCC. ..

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Transfection:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: .. Materials and Methods SP2/O mouse myeloma cells were stably transfected with a construct encoding a λ6 LC (Wil) under control of the CMV promoter, while λ2-producing myeloma cell line RPMI 8226 was purchased from the ATCC. ..

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Construct:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: .. Materials and Methods SP2/O mouse myeloma cells were stably transfected with a construct encoding a λ6 LC (Wil) under control of the CMV promoter, while λ2-producing myeloma cell line RPMI 8226 was purchased from the ATCC. ..

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Control:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: .. Materials and Methods SP2/O mouse myeloma cells were stably transfected with a construct encoding a λ6 LC (Wil) under control of the CMV promoter, while λ2-producing myeloma cell line RPMI 8226 was purchased from the ATCC. ..

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Electroporation:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Modification:

    Article Title: Inhibition of pathologic immunoglobulin free light chain production by small interfering RNA molecules
    Article Snippet: For each set, data were pooled and analyzed for significance by One-Way ANOVA using Dunnett’s Multiple Comparisons test (GraphPad Prism, GraphPad Software Inc., La Jolla, CA, USA). .. SP2/O mouse myeloma cells (American Type Culture Collection; ATCC, Manassas, VA, USA) were stably transfected by electroporation using a construct encoding the V, J, and C regions of a λ6 LC designated Wil [ 13 ] under control of the cytomegalovirus promoter; the modified cells were termed SP2/O-λ6. .. The human λ2 LC producing myeloma cell line RPMI 8226 [ 14 ] was purchased from the ATCC (No. CCL 155), cultured in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 5% FBS/1% penicillin/streptomycin, and maintained in a humidified incubator at 37°C under 5% CO 2 .

    Injection:

    Article Title: Anti-CXCR3 antibodies and methods of use thereof
    Article Snippet: .. The day after the last injection, mice were sacrificed and the spleen was removed and placed in approximately 10 ml of serum-free DMEM (Gibco) in a Petri dish, Sp2\O mouse myeloma cells (ATCC CRL-1581) were fused with spleen cells from the immunized mouse using 50% (w/w) PEG based on the method of Kohler and Milstein (Nature, 256:495-7, (1975)). .. At the end of the procedure the cells were resuspended in 50 ml of ClonaCell-Hy Hybridoma Recovery medium (StemCell Technologies), transferred to a T75 cm2 flask and incubated for 16-24 hrs at 37° C. Following this incubation the cells were harvested and added to 100 ml of ClonaCell-HY methylcellulose selection media (StemCell Technologies).



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